England will be without prop Paul Hill and centre Joe Marchant for their two-Test tour of Argentina after suffering injuries this week.
Forward Hill has aggravated an ongoing neck injury and will remain at the Northampton Saints for rehabilitation while young centre Marchant has suffered a toe injury.
Head coach Eddie Jones has not yet called up replacements but will confirm the final squad for the trip to Argentina on Monday, the day after they play the Barbarians at Twickenham.
England squad to face Barbarians
Forwards: Josh Beaumont (Sale Sharks), Rob Buchanan(Harlequins), Will Collier (Harlequins), Ben Curry (Sale Sharks), Tom Curry (Sale Sharks), Tom Ellis (Bath Rugby), Charlie Ewels (Bath Rugby), Jamal Ford-Robinson (Bristol), Ellis Genge (Leicester Tigers), Ross Harrison (Sale Sharks), Nick Isiekwe (Saracens), Chris Robshaw (Harlequins), Nick Schonert (Worcester Warriors), Jack Singleton (Worcester Warriors), Will Spencer (Worcester Warriors), Sam Underhill (Bath Rugby), Mark Wilson (Newcastle Falcons)
Backs: Mike Brown (Harlequins), Danny Care (Harlequins), Nathan Earle (Saracens), George Ford (Bath Rugby), Mike Haley (Sale Sharks), Sam James (Sale Sharks), Alex Lozowski (Saracens), Jonny May (Gloucester Rugby), Ryan Mills (Worcester Warriors), Richard Wigglesworth (Saracens), Marland Yarde (Harlequins)